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arabisdopsis (v4) gene expression microarray 4×44k chips  (Agilent technologies)


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    Agilent technologies arabisdopsis (v4) gene expression microarray 4×44k chips
    Identification of AtCAPE1 in <t>Arabidopsis</t> . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).
    Arabisdopsis (V4) Gene Expression Microarray 4×44k Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arabisdopsis+(v4)+gene+expression+microarray+4%C3%9744k+chips/pmc04526916-76-1-0
    Average 90 stars, based on 1 article reviews
    arabisdopsis (v4) gene expression microarray 4×44k chips - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis"

    Article Title: A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis

    Journal: Journal of Experimental Botany

    doi: 10.1093/jxb/erv263

    Identification of AtCAPE1 in Arabidopsis . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).
    Figure Legend Snippet: Identification of AtCAPE1 in Arabidopsis . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).

    Techniques Used: Sequencing, Liquid Chromatography with Mass Spectroscopy, Construct, Over Expression, Molecular Weight, Transgenic Assay, Derivative Assay, Western Blot, Staining



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    arabisdopsis (v4) gene expression microarray 4×44k chips  (Agilent technologies)
    90
    Agilent technologies arabisdopsis (v4) gene expression microarray 4×44k chips
    Identification of AtCAPE1 in <t>Arabidopsis</t> . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).
    Arabisdopsis (V4) Gene Expression Microarray 4×44k Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arabisdopsis+(v4)+gene+expression+microarray+4%C3%9744k+chips/pmc04526916-76-1-0
    Average 90 stars, based on 1 article reviews
    arabisdopsis (v4) gene expression microarray 4×44k chips - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification of AtCAPE1 in Arabidopsis . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).

    Journal: Journal of Experimental Botany

    Article Title: A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis

    doi: 10.1093/jxb/erv263

    Figure Lengend Snippet: Identification of AtCAPE1 in Arabidopsis . (A) Deduced amino acid sequence of the PROAtCAPE1 product. The predicted secretion signal peptide is underlined. The CAP domain is shaded in grey. The putative AtCAPE1 peptide is shaded in red. The cleavage site predicted to produce AtCAPE1 is indicated with an arrowhead. The putative cleavage signal motif is double-underlined. (B) LC-MS/MS spectrum of the synthetic AtCAPE1. The y-ion is the C-terminal fragments after peptide bond cleavage while the b-ion is the N-terminal fragments. (C) LC-MS/MS spectrum of the identified AtCAPE1 in Arabidopsis . (D) Schema representing the construct used for constitutive overexpression of enhanced yellow fluorescent protein (eYFP)-tagged PROAtCAPE1. The green box shows the CNYx motif. The putative CAPE is shown in red. The numbers indicate the predicted molecular weight of precursor protein tagged with eYFP (45.7kDa) and the cleaved precursor tagged with eYFP (26.3kDa). (E) Production of the precursor PROAtCAPE1 and the cleaved PROAtCAPE1 in CAPE1ox CNYD and CAPE1ox CNAD transgenic plants, where eYFP was fused to PROAtCAPE1 containing wild type (CNYD) and the mutated (CNAD) junction sequence, respectively. T3 seedlings derived from independent transgenic lines were sampled for western blotting with anti-GFP antibody. Coomassie blue staining was used for protein loading control. The lower panel shows the presence of the T-DNA insertions in the transgenic plants by genomic DNA PCR with the primer pair 35S-F’ and eYFP-R’ shown in (D).

    Article Snippet: Agilent Arabidopsis (V4) Gene Expression Microarray 4×44k chips were used in this study.

    Techniques: Sequencing, Liquid Chromatography with Mass Spectroscopy, Construct, Over Expression, Molecular Weight, Transgenic Assay, Derivative Assay, Western Blot, Staining